Automated design and build processes can rapidly accelerate work in synthetic biology and Baby Toothbrush metabolic engineering.Here the authors present Galaxy-SynBioCAD, a toolshed for synthetic biology, metabolic engineering, and industrial biotechnology that they use Bobble Head to build and execute Galaxy scientific workflows from pathway desig
An immobilized antibody-based affinity grid strategy for on-grid purification of target proteins enables high-resolution cryo-EM
Abstract In cryo-electron microscopy (cryo-EM), sample preparation poses a critical bottleneck, particularly for rare or fragile macromolecular assemblies and those suffering from denaturation and particle orientation distribution issues related to air-water interface.In this study, we develop and characterize an immobilized Ring antibody-based aff
Enhancing Network Longevity and Mitigating Emulation Attack Through Adaptive Metaheuristic Spectrum Sensing in Cognitive Radio Sensor Network
Cognitive Radio Sensor Network (CRSN) provides effective utilization of spectrum, Party Supplies using dynamic spectrum allocation that incorporates multi-hop clustering and routing algorithms for energy-efficient data delivery.The existing clustering and routing methods modeled for Cognitive Radio Sensor Network, typically operates with the assump
Ataxin-3 plays a role in mouse myogenic differentiation through regulation of integrin subunit levels.
During myogenesis several transcription factors and regulators of protein synthesis and assembly are rapidly degraded by the ubiquitin-proteasome system (UPS).Given the potential role of the deubiquitinating enzyme (DUB) ataxin-3 in the UPS, and the high expression of the murine ataxin-3 homolog in muscle during embryogenesis, we sought to define i